# Core B: Animal Model and Immunotyping Core

> **NIH NIH P01** · CLEVELAND CLINIC LERNER COM-CWRU · 2024 · $372,966

## Abstract

Project Summary
Our preliminary data suggested a shared paradigm for the impact of the STING-IFN-β/TGFβ/IL-17 cytokine
network on tumor progression and responses to therapy, which transcends the tissue origins of each cancer.
Our goal, to unravel the crosstalk and define the paradigms, necessitates standardization of the protocols and
procedures used by each constituent project, in order to unify our approaches in establishing preclinical tumor
models and measuring tumor responses to therapy. Our ability to reach meaningful conclusions critically hinges
on comparable execution of in vivo studies. Furthermore, a common readout shared by all three constituent
projects is the inflammatory status in the TME. While immune-supportive inflammation (CD8, Th1, and dendritic
cells) is associated with favorable responses to immune checkpoint inhibitor therapy, an immune-suppressive
inflammatory environment (immature myeloid cells and fibroblasts) can antagonize anti-tumor immunity.
Accurate detection, characterization and quantification of multiple cell types by flow cytometry and single-cell
sequencing are crucial for assessing the state of intra-tumoral inflammation. A shared infrastructure that enables
reliable analysis of the TME is essential for studying cytokine crosstalk. Taken together, the Animal Model and
Immunotyping Core will be charged with two missions. First, the Core will provide technical and experimental
infrastructure to enable uniform and standard in vivo analyses, including preclinical assessment of cancer
therapeutics and characterization of the TME. Secondly, the Core will function as a hub for interaction among
the constituent projects, including analytics assistance and scientific consultation on analyses of the TME.

## Key facts

- **NIH application ID:** 10910034
- **Project number:** 5P01CA272161-03
- **Recipient organization:** CLEVELAND CLINIC LERNER COM-CWRU
- **Principal Investigator:** THOMAS A. HAMILTON
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $372,966
- **Award type:** 5
- **Project period:** 2022-09-13 → 2027-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10910034

## Citation

> US National Institutes of Health, RePORTER application 10910034, Core B: Animal Model and Immunotyping Core (5P01CA272161-03). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10910034. Licensed CC0.

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