# The role of Chlamydia trachomatis specific HLA-E restricted CD8 T cell responses in clearance of infection

> **NIH NIH R21** · UNIVERSITY OF ALABAMA AT BIRMINGHAM · 2024 · $185,625

## Abstract

PROJECT SUMMARY
Chlamydia trachomatis (CT) infection remains the most prevalent bacterial sexually transmitted infection (STI)
worldwide, with over 120 million new CT infections reported annually. In the US, African Americans and women
are disproportionally affected by CT infection. Although over 1.5 million CT cases were recently reported in the
US, this is likely an underestimation as the above-mentioned vulnerable population experiences inequities in
access to routine healthcare. Women experience a greater burden of CT infection due to many reproductive
complications associated with this infection. To date, prevention and control efforts have not significantly reduced
CT infection rates. In addition, up to 20% of infected persons are reinfected within a year suggesting either a
short lived or suboptimal immunity. In the context of both murine models and human studies, we and others have
previously showed a role of IFN-γ-producing CD4+ T cells in chlamydia clearance and protection against
reinfection. Recent studies in humans have however shown that CD8 T cells could play a role in CT infection as
well. CD8 T cells recognize pathogens in the context of peptides presented by an infected cell via classical MHC-
Ia alleles (in humans, HLA-A, B or C) or non-classical MHC-Ib alleles (in case of humans, HLA-E). Prior work
has shown that HLA-E restricted CD8 T cells (HLA-E/CD8s) exert an important immune-regulatory role in control
of several intracellular bacterial pathogens such as Mycobacterium and Salmonella. The role of HLA-E/CD8s in
CT infection as it pertains to clearance of CT infection is unknown. Two previous human studies examining CD8
T cell responses to CT indirectly demonstrated responses restricted by non-classical HLA-I alleles. However,
the precise nature of this restriction and the role of such cells in disease pathogenesis as it pertains to clearance
of CT infection was not defined. Since HLA-Ia and HLA-Ib (i.e., HLA-E) are ubiquitously co-expressed,
appropriate molecular tools and methodologies are needed to dissect the unique contributions of CD8 T cells
responding to peptides presented via each of these alleles. We have recently generated HLA-E*01:01 and
E*01:03 specific cellular resources that allowed us to identify HLA-E/CD8s and to assess their relevance in the
context of CT infection. Specifically, our preliminary data shows that HLA-E/CD8s producing CD107a/IFN-γ/TNF-
α are preferentially detected in women who clear CT infection. Our overall hypothesis is that HLA-E restricted
CD8 T cells will play an important role in clearance of CT infection in women. In Aim 1, we will determine
if CT-specific HLA-E restricted CD8 T cells play a role in the clearance of CT infection in women. In Aim 2, we
will determine if CT-specific HLA-E restricted CD8 T cells can be primed from CT naïve female donors with no
current or prior CT infection. In summary our completed studies will demonstrate the biological relevance and
functionality of HLA...

## Key facts

- **NIH application ID:** 10910100
- **Project number:** 5R21AI175809-02
- **Recipient organization:** UNIVERSITY OF ALABAMA AT BIRMINGHAM
- **Principal Investigator:** Anju Bansal
- **Activity code:** R21 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $185,625
- **Award type:** 5
- **Project period:** 2023-08-18 → 2026-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10910100

## Citation

> US National Institutes of Health, RePORTER application 10910100, The role of Chlamydia trachomatis specific HLA-E restricted CD8 T cell responses in clearance of infection (5R21AI175809-02). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10910100. Licensed CC0.

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