The Biosafety Level 3 (BSL-3) Practices Core (Core 2) will support the research goals of the Regional Biocontainment Laboratory (RBL) at the Tulane National Primate Research Center (TNPRC) by continuing to ensure a safe and secure environment for work with high-consequence pathogens. To do so requires monitoring and training on standard operating procedures and best practices related to the extensive regulatory measures required for working with select agents, including knowledge of proper biosafety, biosecurity, and incident response as well as suitability and inventory management processes. The proposed BSL-3 Practices Core will oversee proficiency development of staff working in the BSL-3 space and associated building systems and provide exceptional training opportunities to ensure that all individuals using select agents are highly qualified and experienced to do so. The Core will also develop and share learning experiences through the TNPRC virtual platform on select agent, biocontainment facilities management, and quality assurance best practices across the NIAID Biodefense Facilities Network and to other NIH-funded scientists. Through these activities, the BSL-3 Practices Core will supply training and facilitate understanding of the health, safety, and biosecurity measures required for working with high consequence pathogens for biodefense, pandemic readiness/response, and emerging infectious diseases.