# Cellular Imaging Core

> **NIH NIH P01** · UNIVERSITY OF ILLINOIS AT CHICAGO · 2024 · $313,603

## Abstract

ABSTRACT
The overall goal of this Program Project is to test the novel hypothesis that vascular endothelial cells (EC) serve
as an immune active niche in the lungs during injury and repair. The Cellular Imaging Core (Core C) is essential
for accurate identification and tracking of the spatiotemporal organization and expression of specific molecules
in the EC regulating the immune niche. The function of the Core is to provide advanced reagents and imaging
methodologies required for both in vitro and in vivo analysis of immune function and to enable testing of the
hypotheses across the Program. We will provide the tools to address how the active regulation of endothelial
cell-surface receptor signaling and mitophagy-mediated recognition of phagocytes by EC promote innate
immune signaling in the lung. To gain the mechanistic signaling insights needed for the success of each Project,
the Core will provide methods at both the conventional and super-resolution levels to identify and interrogate the
signaling pathways. Using multi-channel microscopy and super-resolution activity imaging, we will study
spatiotemporal signaling induced through internalization of tyrosine phosphorylated sphingosine-1-phosphate
receptor1 (S1PR1) in EC and the role of the EC-expressed ubiquitin ligase CFHR in regulating VE-cadherin
expression and function. These analyses will permit a fine-grained assessment of the time-sequence and spatial
organization of the immune signaling in lung EC and how it impacts lung function. By providing a seamless visual
examination of a wide range of signaling activities in individual EC downstream of above listed EC-receptors
during inflammatory injury and repair, the Core is essential for supporting and testing the molecular and
functional hypotheses advanced by the Projects, and crucial to the Program’s overall success.

## Key facts

- **NIH application ID:** 10914671
- **Project number:** 5P01HL160469-03
- **Recipient organization:** UNIVERSITY OF ILLINOIS AT CHICAGO
- **Principal Investigator:** Gary CH Mo
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $313,603
- **Award type:** 5
- **Project period:** 2022-09-20 → 2027-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10914671

## Citation

> US National Institutes of Health, RePORTER application 10914671, Cellular Imaging Core (5P01HL160469-03). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10914671. Licensed CC0.

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