Summary Core C: Next Generation Sequencing core (NGSC) The next generation sequencing core (NGSC) will be responsible for all next generation sequencing activities by the four projects. All sequencing activities will be performed in Core C. RNA-Seq, ChIP-Seq, ATAC-Seq, Core C will be responsible for 1) performing NGS-assays; 2) generating library for Illumina next-generation sequencing, 3) mapping sequence data to EBV, KSHV, MPyV and human genomes, and 4) providing all follow-up bioinformatics and biostatistics analyses for the overall program. These goals will be accomplished in conjunction with the Penn Genomics, Next-Gen-Sequencing and Bioinformatics Cores, which provides Illumina Next Generation Sequencing as a core service. Final data management and analyses will be made accessible to each project investigator via a password protected shared web-based browser for program investigators. Expertise in all aspects of Next-Gen-Sequencing (NGS), Chromatin immunoprecipitation, and Bioinformatics support will be provided to the projects for the lifetime of the award. The NGS core will be led by experience leaders in administration and technical expertise to ensure that the core will be successful. Dr. Lieberman, is an expert in NGS and is familiar with all aspects of NGS technologies. Dr. Robertson has led many large complex programs, and is familiar with management and coordination of the technical expertise needed for success. Thus, the leadership is familiar with different NGS methodologies and bioinformatics analyses as applied to oncogenic herpesviruses and the human genome. Core C leaders have both provide unique expertise that complement each other. The NGS Core C will facilitate the ability of all members of the Program to standardize methods for sample preparation and for post-sequence bioinformatics analyses. The work will be performed at the Perelman School of Medicine in space adjacent to the Robertson lab, and in conjunction with the Penn Genomics NGS Core, and Bioinformatics core supported in part by the Abramson Cancer Center.