# Methods for inferring and analyzing gene regulatory networks using single-cell multiomics and spatial genomics data > **NIH NIH R35** · COLUMBIA UNIVERSITY HEALTH SCIENCES · 2024 · $406,218 ## Abstract Project Summary/Abstract The study of gene regulation provides fundamental mechanistic knowledge to decipher the development of com- plex biological systems and design treatment strategies for diseases by manipulating cell states. Development of single-cell multimodal and multiomics sequencing technologies allows for profiling of additional epigenomics information, such as cis-regulatory elements, which enables inference of gene regulation beyond simply gene coexpression. However, to more accurately identify gene regulation, computational methods are urgently needed to incorporate both transcriptomics and epigenomics data measured in the same cell. Recent studies show that epigenomics changes may foreshadow gene transcription, indicating that gene regulation is an asynchronous process. A set of regulators may not influence the target gene along the whole continuous biological process but only in a small time window. However, current gene regulatory networks inferred from time series data provide average regulation across time rather than characterizing regulation dynamics. Also, prior evidence shows that cell–cell communication and interaction are present in spatially localized cell populations as a form of regular- ized activity. Further incorporating time-varying developmental trajectories in tissue-spatial landscapes will help categorize the spatial heterogeneity of gene regulation. However, we still lack computational methods to incor- porate spatio-temporal trajectories in gene regulation inference. To close these gaps, my research program will (1) develop computational methods and statistical approaches to infer both static and dynamic gene regulatory networks, (2) leverage the spatial trajectories inferred from multiomics and spatial transcriptomics data to charac- terize spatial gene regulation, and (3) develop control strategies for gene regulatory networks to achieve desired states using multi-sample single-cell multiomics data. My long-term goal is to characterize gene regulation across multiple samples and in spatially separated tissue regions and identify the minimum set of driver genes to manip- ulate cell states (e.g., cell types). Building on my previous experience in gene regulatory network inference and methods development to analyze multi-sample single-cell genomics and epigenomics data, I will develop novel statistical methods to infer gene regulatory networks using multi-sample single-cell multiomics data and char- acterize time-lagged activities. I will build on my recent work on single-cell pseudotime analysis and single-cell spatio-temporal trajectories to infer gene regulation in temporal and spatio-temporal contexts. Further, building on my theoretical research in network control and methods development in analyzing multi-sample single-cell omics data, I will develop control strategies for gene regulatory networks to achieve desired cell states or cell de- velopmental trajectories with a minimum set of driver genes. Finall... ## Key facts - **NIH application ID:** 10916287 - **Project number:** 5R35GM150887-02 - **Recipient organization:** COLUMBIA UNIVERSITY HEALTH SCIENCES - **Principal Investigator:** Wenpin Hou - **Activity code:** R35 (R01, R21, SBIR, etc.) - **Funding institute:** NIH - **Fiscal year:** 2024 - **Award amount:** $406,218 - **Award type:** 5 - **Project period:** 2023-09-01 → 2028-08-31 ## Primary source NIH RePORTER: https://reporter.nih.gov/project-details/10916287 ## Citation > US National Institutes of Health, RePORTER application 10916287, Methods for inferring and analyzing gene regulatory networks using single-cell multiomics and spatial genomics data (5R35GM150887-02). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10916287. Licensed CC0. --- *[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*