PROJECT SUMMARY/ABSTRACT Approximately 70% of all new human immunodeficiency virus (HIV) infections are among populations of sexual and gender minorities (SGM) and are primarily attributable to sexual contact.1 Currently only 26% of the U.S. population who would benefit from HIV preexposure prophylaxis (PrEP) are prescribed this effective biomedical HIV prevention strategy2 that would help achieve the “Ending the HIV Epidemic” (EHE) goals that targets a 90% reduction in new HIV infections by 20303. Biomedical prevention strategies for bacterial sexually transmitted infections (STI) like doxycycline postexposure prophylaxis (DoxyPEP) are a vital component to the successes EHE given the well-known association between STIs and risk for HIV infection4,5. Increases in biomedical prevention depend on the individual and interpersonal factors of the clients accessing care and the clinicians who provide the care6. Understanding these, and numerous other potential factors specific to community and society may better inform the approach to HIV and STI biomedical prevention strategies in the primary care setting. In this mixed methods study, we will leverage group concept mapping and conduct focus groups with identified stakeholder groups (e.g., SGM clients and primary care clinicians) to (1) Describe approaches from SGM client perspectives that facilitate awareness, uptake, and persistence of biomedical prevention (same day PrEP & DoxyPEP) in the primary care setting, (2) describe approaches from primary care clinician perspectives that facilitate awareness, uptake, and persistence of biomedical prevention (same day PrEP & DoxyPEP) in the primary care setting and (3) identify the joint priorities of clients and clinicians (high-feasibility and high-importance) for the integration of same day PrEP and DoxyPEP in primary care. Clients and clinicians have a wealth of knowledge and experience and can provide unique perspectives specific to factors influencing HIV and STI biomedical prevention awareness, uptake, and persistence. Findings will inform the development of culturally appropriate interventions to improve the HIV and STI biomedical prevention strategies in the primary care setting. The proposed research and training plan aligns with the National Institute of Nursing Research Strategic Plan on health equity, systems and models of care, prevention/promotion, and population health. The proposed study builds on the applicant’s knowledge of health equity and disparities, improvement in biomedical HIV and STI prevention, and mixed methods approaches. Training will focus on developing proficiency in mixed methods, building expertise in SGM-affirming health and healthcare approaches for vulnerable populations, and advance the scientific understanding of HIV and STI prevention within sexual and gender minorities through scholarly writing and the dissemination of research findings. The training plan and mentorship team will prepare the applicant for a career as ...