PROJECT SUMMARY: Sepsis is one of the leading sources of morbidity and mortality in the US, impacting nearly 1.7 million adults each year and upwards of 250,000 deaths annually. Septic shock is sepsis characterized by hemodynamic failure, with exceptionally high mortality ranging from 15-70%. Septic shock management involves treatment with antibiotics, fluids, and vasopressor therapy. The latter, while effective at hemodynamic support at high doses, is related to major side-effects including peripheral ischemia leading to injury or loss of extremities (toes, fingers, etc.), kidney injury, liver shock, multiple-organ failure. Thus, it is common that survivors of septic shock have a high degree of morbidity due to these side-effects. The goal of this proposal is to validate a new, minimally invasive approach to producing the beneficial effects of vasopressor therapy without the side-effects. We propose that left stellate ganglion nerve stimulation (SGNS) can be used to aid in hemodynamic support during septic shock, and therefore can markedly reduce, or even obviate, the need for high-dose vasopressor administration. To make this clinically practical, we have developed a novel, minimally invasive percutaneous approach to left stellate ganglion nerve stimulation that is controlled via a closed-loop system that is capable of altering stimulation automatically to changes in hemodynamic and other bio-markers (e.g., arterial pressure, heart rate). This project will be completed in several sequential stages. For this Phase I SBIR we will demonstrate proof of physiological concept that left stellate ganglion nerve stimulation in a known sepsis model can 1) provide and sustain a minimum degree of hemodynamic support (at least 15 mmHg for 30 minutes in mean arterial pressure), 2) that direct SGNS lowers vasopressor requirements compared to control by 50%. Separately, we will show that percutaneous SGNS using our proprietary system is safe, feasible and equivalent to direct SGNS in management of septic shock. Successful completion of the specific aims will lead to the development of a clinical-grade system (stimulator controller and percutaneous lead) that can be validated and verified for Phase II of the project and evaluated clinical in an early feasibility IDE trial.