# Development of heparan sulfate-based therapeutics to treat inflammatory diseases > **NIH NIH R44** · GLYCAN THERAPEUTICS CORPORATION · 2024 · $1,463,275 ## Abstract Abstract More than 60 million Americans consume acetaminophen (APAP) on a weekly basis. Unfortunately, an overdose of APAP causes liver toxicity and is responsible for over half of acute liver failure cases in the US. N-acetyl cysteine is the only antidote for APAP overdose and is effective if given within 10 hours after APAP ingestion. However, many patients don’t seek out treatment during the therapeutic window for N-acetyl cystine. Currently, a liver transplant is the only available treatment option for these late-presenting patients. APAP toxicity is a leading cause for liver transplantation in the US and worldwide. The goal of this SBIR phase II project is to develop an anti-inflammatory synthetic heparan sulfate oligosaccharide, GLY-202, for APAP overdose patients specifically late-presenting patients. GLY- 202 was selected after compound optimization studies in efforts to identify a smaller, easier to synthesize oligosaccharide with in vivo efficacy compared to 18-mer in the APAPoverdose model. Unlike 18-mer, the synthesis of GLY-202 can be achieved in a shorter synthetic route, substantially decreasing the production cost and reducing a significant commercialization barrier. This phase II period will focus on IND-enabling studies including GLY-202 drug substance chemical, manufacturing and control (CMC) activities (Aim 1), pharmacology (Aim 2) and toxicity studies (Aim 3). Execution of the proposed aims will demonstrate synthetic scalability and reproducibility in pilot production scale and efficacy and safety through pharmacology and toxicity studies. During the phase II period, Glycan Therapeutics will submit an Orphan Drug Designation application. Benefits of Orphan Drug status include significant financial benefits through fee waivers and tax credits for clinical expenses. Furthermore, the clinical trial recruitment will be comparatively small meaning that the amount of GLY-202 required can be sufficiently prepared by Glycan Therapeutics. In the phase IIb studies, we will complete GMP manufacturing, formulation, submit IND application and conduct Phase 1 clinical trials. The success of this project will provide a new approach to treat drug induced liver toxicity by targeting to HMGB1-mediated inflammation with a first-in-class therapeutic. ## Key facts - **NIH application ID:** 10922837 - **Project number:** 5R44GM144019-03 - **Recipient organization:** GLYCAN THERAPEUTICS CORPORATION - **Principal Investigator:** Zhangjie Wang - **Activity code:** R44 (R01, R21, SBIR, etc.) - **Funding institute:** NIH - **Fiscal year:** 2024 - **Award amount:** $1,463,275 - **Award type:** 5 - **Project period:** 2021-09-14 → 2026-07-31 ## Primary source NIH RePORTER: https://reporter.nih.gov/project-details/10922837 ## Citation > US National Institutes of Health, RePORTER application 10922837, Development of heparan sulfate-based therapeutics to treat inflammatory diseases (5R44GM144019-03). Retrieved via AI Analytics 2026-05-27 from https://api.ai-analytics.org/grant/nih/10922837. Licensed CC0. --- *[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*