# Production of Pedigreed SPF Rhesus Macaques

> **NIH NIH U42** · UNIVERSITY OF CALIFORNIA AT DAVIS · 2024 · $1,190,470

## Abstract

Overall Abstract
The CNPRC has maintained a pedigreed colony of Indian-origin rhesus macaques for over 22 years. The SPF
Pedigreed Indian origin rhesus colony includes both an SPF level 1 colony negative for Simian Retrovirus
(SRV), Simian Immmunodeficiency Virus (SIV), Simian T Cell Lymphotrophic Virus (STLV) , and Herpes B virus
(B virus) and an SPF Level 2 colony free of three additional viruses including rhesus cytomegalovirus
(RhCMV), simian foamy virus (SFV), and rhesus rhadinovirus (RRV). This application will expand the
percentage of SPF Level 2 rhesus while maintaining the current overall colony size. We will continue pedigree
determination for all colony animals and characterize the Major Histocompatibility Complex (MHC) Class I
(Mamu-A and -B) and Class II (Mamu-DRB, -DQA, -DQB, -DPA, and -DPB) loci in our breeding SPF rhesus
macaques. This application will continue to collect fecal microbiome and peripheral blood lymphoid cells as a
biorepository for use by HIV/AIDS investigators. The four principal goals of this application are:
Continue to expand the SPF Level 2 population relative to Level 1 while maintaining overall colony size.
Develop improved and expanded screening and confirmatory tests for the detection of SPF agents to facilitate
the maintenance of the SPF colony. We will initiate annual surveillance for Trypanosoma cruzi in recognition of
the expansion of the geographic range of this parasitic infection. This work includes new assays for agents
such as tuberculosis, which represents the first SPF agent.
Maintain genetic analysis to determine the pedigree of Indian rhesus born into the colony and test all adult
breeders to characterize their Major Histocompatibility Complex (MHC) Class I (Mamu-A and -B) and Class II
(Mamu-DRB, -DQA, -DQB, -DPA, and -DPB) loci.
Continue the collection and storage of intestinal microbiome and peripheral blood lymphocyte samples in a
unique SPF Level 2 biorepository to facilitate future research projects investigating the interaction of HIV with
the host microbiome and immune system.

## Key facts

- **NIH application ID:** 10924916
- **Project number:** 2U42OD010990-24A1
- **Recipient organization:** UNIVERSITY OF CALIFORNIA AT DAVIS
- **Principal Investigator:** Jeffrey A Roberts
- **Activity code:** U42 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $1,190,470
- **Award type:** 2
- **Project period:** 2000-09-30 → 2028-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10924916

## Citation

> US National Institutes of Health, RePORTER application 10924916, Production of Pedigreed SPF Rhesus Macaques (2U42OD010990-24A1). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10924916. Licensed CC0.

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