# MHC Typing Core

> **NIH NIH U42** · UNIVERSITY OF CALIFORNIA AT DAVIS · 2024 · $109,281

## Abstract

Genetics Abstract
The CNPRC has maintained a pedigreed colony of Indian-origin rhesus macaques for over 20 years. The SPF
Pedigreed Indian origin rhesus colony includes both an SPF level 1 colony negative for Simian Retrovirus
(SRV), Simian Immmunodeficiency Virus (SIV) and Simian T Cell Lymphotropic Virus (STLV), Herpes B virus
(B virus) and an SPF level 2 colony free of three additional viruses including include rhesus cytomegalovirus
(RhCMV), simian foamy virus (SFV), and rhesus rhadinovirus (RRV). All colony animals have had MHC typing
for Mamu-A*01, Mamu-A*08, and Mamu-B*01, and the colony is managed to optimize genetic diversity. This
proposal will expand the characterization of the Major Histocompatibility Complex (MHC) to include Class I
(Mamu-A and -B) and Class II (Mamu-DRB, -DQA, -DQB, -DPA, and -DPB) loci. To genotype these markers,
the U42 Genetic Typing Core will submit samples from our adult SPF rhesus macaques to the Genetics
Services Unit at the WNPRC. The application proposes to. The four principal goals of this application are:
Continue to expand the SPF Level 2 population relative to Level 1 while maintaining overall colony size.
Develop improved and expanded screening and confirmatory tests for detecting SPF agents to facilitate the
maintenance of the SPF colony. We will initiate annual surveillance for Trypanosoma cruzi in recognition of the
expansion of the geographic range of this parasitic infection. This work includes new assays for agents such as
tuberculosis, representing the first SPF agent.
Maintain genetic analysis to determine the pedigree of Indian rhesus macaques born into the colony and test
all adult breeders to characterize their Major Histocompatibility Complex (MHC) Class I (Mamu-A and -B) and
Class II (Mamu-DRB, -DQA, -DQB, -DPA, and -DPB) loci.
Continue collecting and storing intestinal microbiome and peripheral blood lymphocyte samples in a unique
SPF Level 2 biorepository to facilitate future research projects investigating the interaction of HIV with the host
microbiome and immune system.

## Key facts

- **NIH application ID:** 10924919
- **Project number:** 2U42OD010990-24A1
- **Recipient organization:** UNIVERSITY OF CALIFORNIA AT DAVIS
- **Principal Investigator:** Jeffrey A Roberts
- **Activity code:** U42 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $109,281
- **Award type:** 2
- **Project period:** 2000-09-30 → 2028-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10924919

## Citation

> US National Institutes of Health, RePORTER application 10924919, MHC Typing Core (2U42OD010990-24A1). Retrieved via AI Analytics 2026-05-27 from https://api.ai-analytics.org/grant/nih/10924919. Licensed CC0.

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