GENOME ENGINEERING CORE - ABSTRACT The goal of the Genome Engineering Core (GEC) of the Washington University Rheumatic Diseases Research Resource-based Center (WU-RDRRC) is to provide access to innovative genomic technologies to build tools that will facilitate pre-clinical modeling and functional testing for the investigation of inflammatory and autoimmune disease. Genomic investigation of the basis of rheumatic disease has become widespread. However, the translation of genetic findings can be challenging, requiring development of models to interrogate basic questions of cellular and disease phenotype and function due to identified genetic changes. This requires development of validated models through innovative genome engineering technologies. These technologies, such as development of genetically-engineered mice and CRISPR/Cas9 editing of induced pluripotent stem cells (iPSCs) and mouse embryos require significant experience and highly-specialized equipment not available within most laboratories. The GEC provides WU-RDRRC investigators with access to the latest in genomic engineering techniques by leveraging services from the Transgenic and Knockout Mouse Facility (TKMF) and the Genome Engineering & Stem Cell Center at McDonnell Genome Institute (GESC), with education and leadership to facilitate projects. We propose to continue these services and innovate the GEC through following three Specific Aims: 1. Sustain, develop, and introduce new state-of-the-art genetics services to WU-RDRRC members. 2. Assist investigators in the generation of genetically engineered mouse models by partnering with the Transgenic and Knockout Mouse Facility (TKMF). 3. Assist investigators in the generation of reagents for creation of genetically-engineered cell lines, iPSCs, and mouse models by leveraging the Genome Engineering & Stem Cell Center at McDonnell Genome Institute (GESC).