Abstract – User Training and Outreach It is imperative that the users of ALS-ENABLE have the training and information necessary to immediately and effectively use the mature synchrotron technologies of the Resource. The User Training and Outreach component of the ALS-ENABLE Resource ensures that individual broad-based training provides a strong basis for users on the operations and best practices of data collection at ALS-ENABLE beamlines. Safety is of the utmost importance and begins before beamtime is scheduled: users are required to complete the ALS Experimental Safety Assessment Form (ESAF) describing their samples and the presence of any toxic or hazardous materials which is then reviewed by the beamline scientist and the ALS Biological Safety Officer. Prior to beamtime users receive training from the ALS on the proper use of PPE, cryogen handling, emergency procedures, storage and safe use of chemicals, and laboratory safety (if applicable). At the beamline, staff provide on the job training on the safe, efficient use of beamline hardware. Additionally, a multifaceted approach that incorporates face-to-face onsite training, the use of online and remote materials, and group training is used to help users achieve their goals. Workshops and seminars will train large groups at once and our unique “reverse” synchrotron trip provides training at the NIH user’s home institution. Outreach activities are a fundamental component of informing the scientific community of the capabilities, availability, and modes of access to the ALS-ENABLE Resource. We will continue to publish results in peer-reviewed journals, participate in and host meetings and workshops, and strive to reach out to under-represented communities to promote the Resource, recruit new users, and engage the structural biology community. Specific efforts will be made to engage and recruit projects from scientists that may not use structural biology as their main technique. A robust web presence (als-enable.lbl.gov) supports these efforts and provides access to all beamline manuals, training materials, scientific highlights, and descriptions of the technologies available in the different ALS-ENABLE cores.