Technology Operations Core 1: Abstract Technology Operations Core 1 (TOC1) of the ALS-ENABLE resource supports the rapid response to macromolecular crystallography diffraction data collection. It joins forces, and groups together the effort and expertise of three world-leading groups operating macromolecular crystallography beamlines at the ALS. They are the Berkeley Center for Structural Biology (BCSB), the Molecular Biology Consortium (MBC) and the University of California at San Francisco. The ALS-ENABLE facility includes eight macromolecular crystallography beamlines with a broad range of capabilities to address all structural biology projects. NIH investigators can request beamtime and, following a positive review, will get beamline access within a month. Three modes of access are made available to users under the rapid response in TOC1. The vast majority of users take advantage of the remote access capabilities available at all beamlines. Users ship their samples and the ALS-ENABLE staff will prepare the beamline and load users’ samples in the robotic system. The investigators will then operate the beamline from their home or institution for the acquisition of diffraction data. A new mode of access proposed in this effort is to use the fully automated data collection pipeline. Here, diffraction data are acquired automatically without manual intervention. This has been exploited heavily in fragment-based drug discovery campaigns in the fight against COVID-19 pandemic. The automated data collection pipeline has shown to be powerful and acquire diffraction datasets on crystals that appears to be unusable. Users can also rely on the collaborative crystallography programs. More often novice structural biologists will need help in their effort to determine the structure of biological molecules. Collaborative crystallography relies on expert scientists available from the ALS-ENABLE staff to collect diffraction data on their behalf. The range of provided expertise can start with guidance with crystallization up to publication of the results. The collaborative crystallography program will also support the automated data collection pipeline by identifying challenging projects that may need manual intervention. This will ensure that users are getting the best diffraction data from their precious samples. Altogether TOC1 intends to quickly respond to the need of macromolecular crystallography NIH investigators for access to diffraction data at the ALS.