PROJECT SUMMARY (Multi-omics Core) Single-cell transcriptomics is a central component of the PRECISION Human Pain Network because these powerful new tools provide unprecedented opportunities for understanding the cell types, molecules, and cell- cell interactions that contribute to chronic pain. The Multi-omics Core will be led by Dr. Renthal and centralizes the single-cell transcriptomic, epigenomic, and next-generation sequencing efforts with an experienced team in both the development, optimization, and analysis of single-cell and next-generation sequencing efforts. The centralization of these cutting-edge technologies will ensure that the established protocols can be applied systematically to all samples from the Center. Specifically, the Core will provide single-cell and single-nucleus multi-omic services to characterize pain-related tissues in both Projects 1 and 2. They will then provide both Projects rapid next-generation sequencing services with 1-3 day turnaround times at cost. Finally, the Core will remain at the cutting-edge of the rapidly evolving field of single-cell genomics and continue to bring innovations that improve quality and reduce costs back to the Center. The Core will also coordinate with similar Cores across the U19 PRECISION Human Pain Network to reconcile protocols and best practices.