# DMS/NIGMS 1: Multiscale modeling of Notch signaling during long-range lateral inhibition

> **NIH NIH R01** · CLARKSON UNIVERSITY · 2024 · $196,114

## Abstract

The spatiotemporal distribution of morphogens contributes to the organized development of tissues and organs. 
One model of morphogen distribution is active transport, which includes cell based mechanisms like signaling 
filopodia. Signaling filopodia facilitate contact between distant cells in order to allow signaling to occur, and support 
several cell signaling paradigms during development. The proposed project will use multi-scale modeling and 
biological experiments to test the hypothesis that Notch signaling occurs via filopodia-filopodia mediated cell-cell 
contacts in vivo. This hypothesis will be tested in three objectives. (1) Investigate the mechanism of Notch activation 
on filopodia. A mechanical model of distinct modes of filopodia interactions will be used to quantify the forces 
generated during filopodia mediated signaling to identify the most likely mechanism for Notch activation. (2) 
Determine how Notch signal is relayed to the cell body. A mathematical model of filopodia in the presence of 
diffusion and active transport of signals will be developed to quantify the relative importance of each mechanism. 
We will support our model with genetic approaches and quantitative live imaging. (3) Create a multi-scale vertex 
model of Notch signaling during bristle cell patterning. We will combine the above molecular and cellular submodels of Notch signaling to create a truly multi-scale vertex model of the patterning thorax. This framework will 
support an in silico, real-time investigation of patterning dynamics via signaling filopodia to identify potential 
molecular regulators of this process. The success of this proposal will result in a foundational understanding of the 
mechanisms that drive long-range lateral inhibition during tissue patterning. We will introduce the first multi-scale 
mechanical model of the fly thorax that allows for cell-driven dynamics of filopodia and real-time activation of 
Notch. The experimental work proposed here addresses a major gap in our understanding of tissue development 
and homeostasis: how active cell processes contribute to the distribution and activation of signals.

## Key facts

- **NIH application ID:** 10932403
- **Project number:** 5R01GM152810-02
- **Recipient organization:** CLARKSON UNIVERSITY
- **Principal Investigator:** Emmanuel Asante-Asamani
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $196,114
- **Award type:** 5
- **Project period:** 2023-09-25 → 2026-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10932403

## Citation

> US National Institutes of Health, RePORTER application 10932403, DMS/NIGMS 1: Multiscale modeling of Notch signaling during long-range lateral inhibition (5R01GM152810-02). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10932403. Licensed CC0.

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