This proposal aims to continue to improve the quality and breadth of predoctoral immunology training at Dartmouth. It builds upon and improves a continually funded T32 program that has been successfully training the next generation of immunologists for thirty years. The scientific focus is to better understand the immune system in health and disease and to leverage these findings to develop better therapies for an array of different conditions where the immune response is central to pathogenesis. Our goal is to provide a cutting-edge program to train the best graduate students to conduct rigorous and ethical research in the field of immunology. Trainees will be mentored to become productive researchers who are experts in their fields and also very well-informed about immunology as whole. Faculty trainers are drawn from a wide variety of areas within immunology. The program can therefore offer interdisciplinary training that includes the interfaces between immunology and infectious disease, cancer, mucosal homeostasis, pulmonary disease, autoimmunity and systems biology. In addition to training in the design, implementation, reproducibility and interpretation of research projects, the program teaches students how to effectively present their research in seminar presentations and in written formats such as grant proposals. Major strengths of the program include: (i) the strength of the research programs of the training faculty. Faculty research program represent a broad array of disciplines within immunology, many of which span different fields and involve interdisciplinary collaborations with different labs both within Dartmouth and at other institutions. The relatively small size of Dartmouth laboratories also leads to very close interactions between faculty and trainees, creating an ideal learning environment. (ii) Students appointed to the training program enter through two very strong umbrella graduate programs, the Molecular and Cellular Biology program and the Quantitative Biological Sciences program. Graduate education is coordinated by the Guarini School of Graduate and Advanced Studies, that offers a wealth of professional development activities for students, supplemented by activities by training program trainers, to help them with their future careers. (iii) The Geisel School of Medicine and Thayer School of Engineering at Dartmouth offer state-of-the- art facilities for laboratory research, computational and statistical resources, an AAALAC approved animal facility and core facilities for genomics and bioinformatics. In this competitive renewal we detail new training programs that have been added during the last grant period and new initiatives we propose adding to the training program. The Dartmouth Immunology Program has had outstanding track record of success in training young immunologists over the past thirty years, and trainees populate many of the most prominent immunology laboratories throughout the world. We will augment this ...