# Protein Production, Structural Biology, and Nucleic Acid Sequencing and Analysis

> **NIH NIH P01** · VERSITI BLOOD HEALTH, INC. · 2024 · $412,500

## Abstract

CORE B SUMMARY
 This Program Project focuses on a group of closely related and clinically important antibody-mediated
thrombocytopenias in which β3 integrins (αIIbβ3, αVβ3, and single-chain β3 fragments) and platelet chemokines
including platelet factor 4 (PF4) and platelet basic protein isoforms (PBPi) are among the target antigens. Purified
antibodies of human and mouse origin, and protein antigens including integrins, PF4, PBPi, and their mutants
represent essential materials shared among the three Projects for the proposed structural and functional studies.
In addition, next-generation sequencing (NGS) is a powerful tool that will be utilized by Projects 2 and 3 to
isolate anti-PF4 and anti-PBPi monoclonal antibodies, and investigate the molecular and cellular mechanisms
underlying the ontogeny of B cells that generate harmful antibodies resulting in antibody-mediated thrombocyto-
penia and associated pathogenesis. The overall goal of Core B is to provide centralized and standardized
large-scale antibody and protein production and authentication services, structure-based data interpretation of
antibody-antigen interaction and functional assays, as well as centralized NGS and data analysis to each of the
Projects in our Program, which should reduce the time and effort, maximize cost savings, ensure data repro-
ducibility, cross-validation, and interpretation in parallel and/or complementary studies.

## Key facts

- **NIH application ID:** 10934142
- **Project number:** 1P01HL167668-01A1
- **Recipient organization:** VERSITI BLOOD HEALTH, INC.
- **Principal Investigator:** Jieqing Zhu
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $412,500
- **Award type:** 1
- **Project period:** 2024-08-01 → 2029-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10934142

## Citation

> US National Institutes of Health, RePORTER application 10934142, Protein Production, Structural Biology, and Nucleic Acid Sequencing and Analysis (1P01HL167668-01A1). Retrieved via AI Analytics 2026-06-02 from https://api.ai-analytics.org/grant/nih/10934142. Licensed CC0.

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