# Organizing and Reorganizing Human Testis Development In Vitro

> **NIH NIH R01** · MAGEE-WOMEN'S RES INST AND FOUNDATION · 2024 · $523,911

## Abstract

ABSTRACT
This is a multi-PI, multi-institutional grant to develop physiomimetic systems that mimic human testis
development and spermatogenic lineage development, ex vivo. Chemotherapy and radiation treatments for
cancer or gender affirming treatments for transgender patients can cause infertility. Adult patients have the
option to cryopreserve eggs or sperm prior to treatment that can be thawed in the future and used to achieve
pregnancy with standard assisted reproductive technologies, including IVF. Those options are not available to
all adult patients or to prepubertal patients who are not able to produce mature eggs or sperm. Although
prepubertal boys or transgender girls who are on gender affirming treatments are not producing sperm, they do
have spermatogonial stem cells SSCs in their testes that have the potential to produce sperm. The MPIs in
Chicago and Pittsburgh have been cryopreserving immature testicular tissues for young patients for more than
a decade with anticipation that those tissues can be thawed in the future and matured to produce sperm. Each
patient donates a portion of their tissue to research to develop next generation technologies that will allow
them to use their tissues for reproduction. We will use those tissues to gain fundamental insights into human
testis development from newborn to adult stages of life. This will establish benchmarks to evaluate our
progress engineering three novel testis physiomimetic systems that support spermatogenesis, ex vivo. Aim 1
will replicate mouse testicular tissue organotypic culture at the air/liquid interface using a microfluidics device
and a static PDMS roof-transwell system before translating the approach to human testis tissues. Aim 2 will
establish mouse and human testicular organoids, which will then be induced to fuse into elongated
seminiferous tubule-like structures. Aim 3 will print seminiferous tubule like scaffolds using human testis
extracellular matrix (htECM) and pig testis ECM (ptECM) hydrogels as bioink and then seed the scaffolds with
mouse and human testicular somatic cells and germ cells. These testicular physiomimetic systems will provide
fundamental insights into human testicular somatic cell and germ cell development and establish experimental
platforms to test toxicologic or pharmaceutical compounds or drugs that promote or prevent sperm production.
The overarching objective is to mature human testicular tissues in one or more of these physiomimetic systems
to produce fertilization competent sperm, ex vivo.

## Key facts

- **NIH application ID:** 10934359
- **Project number:** 5R01HD114210-02
- **Recipient organization:** MAGEE-WOMEN'S RES INST AND FOUNDATION
- **Principal Investigator:** Monica M Laronda
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $523,911
- **Award type:** 5
- **Project period:** 2023-09-22 → 2028-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10934359

## Citation

> US National Institutes of Health, RePORTER application 10934359, Organizing and Reorganizing Human Testis Development In Vitro (5R01HD114210-02). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10934359. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*