The Nebraska Center for the Prevention of Obesity Diseases through Dietary Molecules (NPOD) leveraged NIGMS Renovations and Alteration funds ($300,000) and institutional support to develop a new Research Core (Biomedical and Obesity Research Core, BORC) at the University of Nebraska-Lincoln (UNL) in Phase 1. NPOD's unique niche in obesity research is the study of bioactive food compounds that prevent, treat, and cure obesity and co-morbidities. BORC is a critical resource to enable this work in Nebraska and has expanded considerably over the Center's first nine years. In Phases 1 and 2, NPOD leadership negotiated $470,092 renovation funds and 3,353 square feet in space and acquired equipment worth $3,311,369 through UNL commitments, equipment donations, and four administrative supplements (equipment) from NIGMS. BORC is now comprised of the Obesity and Nutrition Laboratory, Small Animal Research Laboratory, and Microbiome Suite. Together, these provide access to equipment unique to core research facilities located in Nebraska and support services that include consultation on experimental design, data acquisition, large data processing, and biostatistical analysis. BORC works with NPOD's Administrative Core to coordinate services provided by NPOD's biostatisticians and is networked to the Holland Computer Center, UNL's supercomputing facility. BORC has 94 accounts (principal investigators), representing 256 users (12 external), and numbers of samples analyzed and service requests received have more than doubled from the end of Phase 1 (2019) to the end of Year 9 in Phase 2. BORC has analyzed a cumulative 151,163 samples through an annual average of 1,400 service requests since 2014. BORC has generated $1,228,197 in service fees since it started charging fees in June 2017 and has generated an average annual revenue of $266,335 since becoming a UNL-approved fee-for-service center in June 2019. Users have expressed their satisfaction with the service through surveys, and UNL has recognized the importance of BORC for biomedical research in Nebraska, committing $200,000 and 507 square feet of new space to BORC in Phase 3, as well as $294,628 toward the director's salary and equipment upgrades in the subsequent three years. BORC is on a strong trajectory for sustainability after Phase 3: BORC user fees ($266,335) cover 84% of BORC operating expenses, and any shortfall can be covered by F&A costs returned to the Center ($173,444 in FY2022). This positions BORC for continued institutional support, as evaluated by reasonable benchmarks such as decreasing subsidy rates to under 10% and supporting 50% of expenditures through user fees. In Phase 3, the goal is to position BORC for sustainability and growth after end of NIGMs funding relative to current levels. NPOD and BORC will seek to meet this goal through the following Specific Aims. Specific Aim 1. Retain existing and recruit new users through acquiring state-of-the-art instrumentation. Specific Aim 2. R...