# National Xenopus Resource

> **NIH NIH P40** · MARINE BIOLOGICAL LABORATORY · 2024 · $302,992

## Abstract

Project Summary
This grant supports a project to generate and characterize Xenopus mutants in key genes related to human
disease, develop new transgenic lines for genome editing and establish efficient site-specific integration. The
long-term goals of the studies are to generate new models of human disease that will provide useful resources
for the biomedical research community. The proposed studies will use CRISPR-Cas gene editing to create
precise models of human disease in the amphibian Xenopus. The current studies are focused on developing
Xenopus models of many different diseases for the entire Xenopus community, and each mutant will be
developed in close coordination with individual researchers. There are four main aims to this proposal. First,
we propose to collaborate with Xenopus researchers to characterize existing mutants (over 200) that were
developed over the last 5 years. The mutants cover a wide range of topics that require varied expertise that
can only be obtained through interactions with other researchers. Second, we propose to generate new
mutants using CRISPR-Cas as requested by researchers. As the national stock center for Xenopus, we have
the expertise to breed and maintain these mutants. Third, we will generate new transgenic lines that will be
used for genome editing. These new lines will allow for more tissue-specific expression of Cas9. Fourth, we
propose to develop homology directed repair for more efficient generation of site-specific integration of
exogenous DNA. All these aims will help enhance the utilization of CRISPR-Cas gene editing methods in the
Xenopus model system.

## Key facts

- **NIH application ID:** 10935150
- **Project number:** 3P40OD010997-15S1
- **Recipient organization:** MARINE BIOLOGICAL LABORATORY
- **Principal Investigator:** Marko E Horb
- **Activity code:** P40 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $302,992
- **Award type:** 3
- **Project period:** 2010-08-12 → 2026-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10935150

## Citation

> US National Institutes of Health, RePORTER application 10935150, National Xenopus Resource (3P40OD010997-15S1). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10935150. Licensed CC0.

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