Core C: Pathology - Abstract The UNC Breast SPORE Pathology and Immunobiology Core (PIC) supports and enhances cancer-related translational, clinical, population and basic science research. The PIC provides SPORE participants with a centralized, coordinated, quality-controlled, quality-assured facility for the procurement, processing, storage, and distribution of human biologic specimens. This facility also acts as the conduit for access to tissues from multiple other groups (UNC-Rex Hospital, East Carolina Brody School of Medicine) and shared resources (Pathology Services Core, Office of Clinical Trials and Research (OCTR), Immune Monitoring and Genomics Facility). The centralized collection services offered by the Core result in a smooth and seamless process for the banking of human tissues and blood for SPORE investigators using clinical specimens for correlative, molecular, immunological, translational, and epidemiological cancer research. Breast cancer-specific efforts that have and will support SPORE aims include centralized tissue and specimen banking, receipt and processing of breast-related human specimens including: freshly procured, snap-frozen and formalin fixed paraffin embedded (FFPE) block specimens, preparation of tissue microarrays (TMAs), cell microarrays, morphological evaluation and morphology-based assays (immunohistochemistry, immunofluorescence, in-situ hybridization, and fluorescence in-situ hybridization), assay development & training, immunogenetics, flow-cytometry, and digital imaging and image analysis for spatial quantification of molecular analytes in intact specimens. Project # 1, CBCS, has used the Core for over two decades with ascertainment of tumor samples from hospitals across North Carolina, demonstrating consistent success with CBCS tumor block accrual of over 95%. Projects #2, #3, and #4 will specifically use the Core to track samples from clinical trials with special provisions for obtaining fresh tissue in protocol-specific procedures. Additional support for Projects #2, #3, and #4 will include procurement of fresh tissue from human and PDX samples to flow sort cells for assays and the identification of regions of interest in tumors and the tumor microenvironment for spatial transcriptomic analysis.