# Reconstructing Somatic Chromosomes

> **NIH NIH R01** · OREGON HEALTH & SCIENCE UNIVERSITY · 2024 · $648,606

## Abstract

Project Summary:
Somatic cell nuclear transfer (SCNT) replaces haploid meiotic chromosomes in metaphase (MII)-arrested
oocytes with diploid interphase chromatin from somatic cells. Such SCNT oocytes are diploid and are not suitable
for fertilization due to triploidy. However, our preliminary studies indicate that SCNT oocytes are capable of
forming premature meiotic spindles consisting of paired single-chromatid chromosomes and subsequent
activation or fertilization can induce segregation of somatic chromosomes into a pseudo polar body. Our research
will determine if somatic homolog pairing and recombination in mouse SCNT oocytes can be induced by DNA
double strand breaks and develop future therapeutic approaches for infertility treatments.

## Key facts

- **NIH application ID:** 10936533
- **Project number:** 5R01HD113562-02
- **Recipient organization:** OREGON HEALTH & SCIENCE UNIVERSITY
- **Principal Investigator:** SHOUKHRAT MITALIPOV
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $648,606
- **Award type:** 5
- **Project period:** 2023-09-26 → 2028-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10936533

## Citation

> US National Institutes of Health, RePORTER application 10936533, Reconstructing Somatic Chromosomes (5R01HD113562-02). Retrieved via AI Analytics 2026-05-27 from https://api.ai-analytics.org/grant/nih/10936533. Licensed CC0.

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