ABSTRACT The goal of the parent project is to understand how social isolation and loneliness affect caregiver burden, understand the momentary role of loneliness in physical health outcomes, and identify temporal targets for intervention based on momentary loneliness in ADRD spousal caregivers. We will examine how vagally mediated HRV impacts mental and physical wellbeing in ADRD spousal caregivers as an exploratory aim. Three Hundred ADRD spousal caregivers will complete assessments on attachment, quality of life, caregiver burden, grief symptoms, and participate in a blood draw to evaluate inflammation and indices of cellular aging (i.e., mitochondrial and glycolytic function). These caregivers will also complete momentary assessments of depressive affect, loneliness, and social isolation, and we will continually measure vagally mediated HRV over two weeks. The present study aims to provide biobehavioral evidence in support of the minority stress model with respect to Latino ADRD spousal caregivers. Aging Latino adults are at increased risk for inflammatory disease, including type 2 diabetes mellitus and ADRD. Chronic stress underlies one mechanism contributing to this increased risk. For example, those caregiving for a spouse with ADRD and experiencing stress from ongoing discrimination may be at increased risk for inflammatory disease. To our knowledge, no studies examine the specific association between compounding caregiving and discriminatory stress and risk for inflammatory disease. We predict that Latino ADRD spousal caregivers will report increased caregiver burden and display increased risk for inflammatory disease (proinflammatory cytokine production). We also predict that these associations will be explained by discriminatory stress. Investigating the role of discrimination may help future researchers develop interventions that may reduce disease burden in Latino ADRD caregivers.