# Stem Cell, Organoid and Cell Phenotyping Module

> **NIH NIH P30** · UT SOUTHWESTERN MEDICAL CENTER · 2024 · $202,222

## Abstract

Resource/Service Core: Stem Cell, Organoid, and Cell Phenotyping Module
Abstract
The goal of the Stem Cell, Organoid and Cell Phenotyping (SOCP) Module is to provide vision researchers
with access to cutting-edge technologies including stem cell and organoid development, extracellular vesicle
analysis, and immunometabolism phenotyping. To accomplish this goal, this Module is structured into two
arms. The first arm will support the development of induced pluripotent stem cells (iPSCs) and organoids
relevant to the eye. The second arm will provide comprehensive services for immunometabolic profiling,
including flow cytometry, high throughput cellular analysis, live cell metabolic profiling using Seahorse and high
resolution respirometry (Oroboros), and analysis of extracellular vesicles from cell culture and human body
fluids. Together, this Module will allow for the rigorous study of a wide range of NEI-focused research activities,
including all NEI-funded research projects, and new collaborative and pilot projects. The director and staff of
this Module will provide training, technical support, and maintenance of key equipment to facilitate scientifically
rigorous, cutting-edge, vision research.
The Stem Cell/Organoid arm of this Module will provide the following essential services:
1) Provide technical support and reagents for the derivation, culture, validation, and cryopreservation
 of induced pluripotent stem cells (iPSCs).
2) Provide technical support for the generation and culture of organoids from human and mouse
 embryonic and adult stem cells.
3) Provide reagents for mycoplasma testing, STR profiling, and karyotyping analysis.
The Cell Phenotyping arm of this Module will provide the following comprehensive analytical services:
1) Flow cytometry
2) Cell sorting
3) High throughput cellular analysis
4) Live cell metabolic profiling
5) Microvesicle quantification and size determination
Collectively, these services will provide our vision research community with state-of-the-art resources that will
increase their experimental efficiency and support the generation of high quality, quantitative data for all
aspects of visual science.

## Key facts

- **NIH application ID:** 10939300
- **Project number:** 2P30EY030413-06
- **Recipient organization:** UT SOUTHWESTERN MEDICAL CENTER
- **Principal Investigator:** DANIELLE M. ROBERTSON
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $202,222
- **Award type:** 2
- **Project period:** 2019-09-30 → 2029-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10939300

## Citation

> US National Institutes of Health, RePORTER application 10939300, Stem Cell, Organoid and Cell Phenotyping Module (2P30EY030413-06). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10939300. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*