Healthy adolescent brain maturation involves several highly coordinated processes including extensive and dynamic reorganization of structural and functional networks and neuropil contraction. However, excessive neuropil contraction, and impaired network reorganization may result in dysconnectivity in structural, diffusion, and functional networks that may explain why more than 50% of psychiatric disorders begin during adolescence. This is particularly notable in first-degree relatives of persons with schizophrenia/schizoaffective disorder (familial high risk for schizophrenia (FHR)) possibly due to additional familial risk. Prior studies on adolescents have examined gray matter (GM) metrics (e.g., volumes) to index neuropil changes and have studied one modality of network, e.g., structural, or functional to elucidate network pathology. However, GM metrics do not specifically index neuropil (cortical regions rich in synapses, dendrites, axonal endings, interneurons, glia, and unmyelinated axons with few cell bodies). Similarly, examining individual network modality (monoplex network) does not reflect concomitant changes in other network modalities. Therefore, we propose to examine neuropil growth/contraction using 3D whole-brain phosphorus magnetic resonance spectroscopy (31P MRS). To elucidate alterations in multiple modalities of networks, we will use a novel multiplex network analysis that provides composite metrics of dysconnectivity in multiple network layers that may be closer to the ‘ground-truth’ of network pathology since networks in the brain tissue are essentially multiplex in nature. Our goal is to longitudinally examine neuropil growth/contraction and multiplex network dysconnectivity in relation to categorical (axis I disorders) and dimensional (severity of cognitive/social deficits, and psychopathology) outcomes using an accelerated cohort design on 210 subjects between the ages encompassing onset of puberty and 18 years equally distributed for the FHR status. Our proposal is strongly supported by convergent preliminary data that show greater psychiatric morbidity, GM loss in the fronto-temporal network (FTN) regions and altered structural, diffusion, and functional networks among adolescent FHR persons, and impairments in selected multiplex network metrics suggesting multiplex network dysconnectivity. We will use ultra-high field neuroimaging at 7 Tesla to obtain high resolution MP2RAGE, 3D whole-brain 31P MRS with superior spectral resolution, diffusion weighted imaging with Neurite Orientation Dispersion Density Imaging (NODDI) modeling, and resting fMRI, and collect psychopathological, cognitive, and social function data at baseline, 1, and 2-years to characterize longitudinal trajectory of neuropil growth/contraction (Aim 1), its relationship to multiplex network dysconnectivity (Aim 2) and with categorical and dimensional outcomes. We will build an integrated model of multiplex network features and neuropil growth/ contractio...