# A high-throughput assay to measure amyloid-β oligomer formation

> **NIH NIH R21** · UNIVERSITY OF WISCONSIN-MADISON · 2024 · $219,930

## Abstract

Project Summary
Toxic oligomers formed by amyloid-beta (Aβ) self-association are of high interest for research
into the causes and treatment of Alzheimer's Disease. Studies Aβ oligomer formation on a
molecular level using biophysical and analytical techniques have provided many valuable
insights, but there remain many open questions regarding oligomer structure and the factors
that influence their formation. In the present proposal, we will develop a new method for
studying Aβ oligomerization that greatly diverges from the biochemical and biophysical
approaches historically utilized by the field. Here, we will use a genetically encoded sensor that
detects Aβ oligomer formation and converts the oligomer formation event to the expression of a
reporter gene, enabling high-throughput and direct measurements of Aβ oligomerization. This
format enables us to measure the effects of millions of different factors on Aβ oligomerization
and to quickly identify agents that can selectively bind oligomers over other Aβ species.
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## Key facts

- **NIH application ID:** 10947549
- **Project number:** 1R21AG088715-01
- **Recipient organization:** UNIVERSITY OF WISCONSIN-MADISON
- **Principal Investigator:** Tina Wang
- **Activity code:** R21 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $219,930
- **Award type:** 1
- **Project period:** 2024-09-15 → 2027-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10947549

## Citation

> US National Institutes of Health, RePORTER application 10947549, A high-throughput assay to measure amyloid-β oligomer formation (1R21AG088715-01). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10947549. Licensed CC0.

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