# Genomic Editing & Screening

> **NIH NIH P30** · SLOAN-KETTERING INST CAN RESEARCH · 2024 · $443,017

## Abstract

ABSTRACT
The Genomic Editing and Screening (GES) Core was established in 2012 with institutional funds. Initially, it
provided cutting-edge RNA interference (RNAi) gene target knockdown technologies toward the discovery of
novel cancer targets and elucidation of complex aberrant intracellular pathways. A central feature is the use of
third generation Pol II-driven, enhanced Mir30-based shRNA for high functional protein knockdown efficiency
at single-copy integration, using a diverse panel of packaged retro-and lentiviral plasmid backbones for
effective delivery. Establishment of this platform subsequently allowed for the rollout in 2014 of CRISPR-Cas9
sgRNA single-gene and full-genome knockout technologies via pooled libraries. Realization that many cell
types are easily transfectable and that discrete intracellular events such as activation, translocation, and cell-
to-cell communication can be quantified using high-speed automated fluorescence microscopy, the Core
initiated high content imaging and analysis (HCA) for arrayed siRNA experimentation. A natural extension of
this 96/384 well plate format was then evolved into a workstation-based small molecule compound screening
program, allowing investigators to effectively phenocopy newly-identified targets using chemical biology
approaches. The most promising of these compounds are then matriculated into the Early Therapeutics Center
(ETC) and Tri-Institutional Therapeutic Drug Initiative (TRI-I TDI). Overall, this multi-modal Core enables
mechanism-based science through target identification and drug screening, offering custom reagents and
services to a growing number of the Center’s members.

## Key facts

- **NIH application ID:** 10953740
- **Project number:** 4P30CA008748-58
- **Recipient organization:** SLOAN-KETTERING INST CAN RESEARCH
- **Principal Investigator:** Ralph J. Garippa
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $443,017
- **Award type:** 4N
- **Project period:** 1997-01-20 → 2025-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10953740

## Citation

> US National Institutes of Health, RePORTER application 10953740, Genomic Editing & Screening (4P30CA008748-58). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10953740. Licensed CC0.

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