Project Summary I am the Technical Director of the Proteomics and Macromolecular Crystallography Shared Resource (PMCSR) at the Herbert Irving Comprehensive Cancer Center (HICCC), Columbia University Irving Medical Center (CUIMC). I joined PMCSR in 2018 and established and expanded the service portfolio. Utilizing cutting-edge mass spectrometers and innovative methodologies, PMCSR has been supporting various research programs, including Cancer Genomics and Epigenomics, Precision Oncology and Systems Biology, Cancer Population Science, and Tumor Biology and Microenvironment. Its impact is evident in the novel immunotherapy targets identified, the deeper understanding of cancer pathogenesis achieved, and the elucidation of key mechanisms driving tumorigenesis. The PMCSR's services have been widely utilized not only within HICCC and Columbia University but also by external cancer centers, highlighting its significance in the broader research community. This widespread usage underscores the PMCSR's exceptional reputation, evident in its outstanding rating during the recent P30 Comprehensive Cancer Center grant renewal. In the past five years, I have been a co-author of 47 peer-reviewed articles. My aim is to enhance PMCSR's capabilities further by advancing existing technologies and introducing innovative methodologies, including Automated Deep Proteomic Profiling for Biofluids, Comprehensive Pipeline for Immunopeptidomics and Cell Surfaceome Profiling, and Lab Automation. The successful implementation of these technological advancements will significantly impact cancer studies, attracting substantial funding, fostering collaborations, and leading to high-impact publications. In summary, this award will provide career stability to me and allow me to continue excellence in serving the cancer research community. The advancements will not only elevate the PMCSR's capabilities but also enrich the broader landscape of cancer research, marking a significant impact toward improved diagnostics, treatments, and ultimately, a cure.