# Transcriptomic and proteomic analyses of Upc2A under Candida glabrata hypoxic growth

> **NIH NIH P20** · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · 2024 · $258,533

## Abstract

Adaptation to hypoxia is crucial for human pathogens like Candida glabrata, since the condition 
mimics the infection environment inside the host. Hence, our long-term goal is to identify 
essential factors that are required for C. glabrata hypoxic growth, and to exploit them for novel 
Candida drug development. However, the transcriptional responses of C. glabrata shifting from 
normoxic respiratory to hypoxic metabolism is unexplored. My recent work has indicated that 
the transcription factor Upc2A is activated hypoxically, and its function is essential for C. 
glabrata hypoxic growth. Although Upc2A roles in C. glabrata drug resistance mechanism is well 
characterized, its intrinsic function in C. glabrata biology remains under-studied. This application 
takes an interdisciplinary approach combining cutting-edge next generation sequencing, 
proteomics, and molecular genetic techniques to determine the fundamental function of Upc2A 
in C. glabrata hypoxic physiology. Our overall objectives are (i) to decode the comprehensive 
interaction networks of Upc2A target genes as well as genes that are required for C. glabrata 
hypoxic adaptation, and (ii) to identify Upc2A-associated proteins required for the activity of this 
transcription factor and the organism’s hypoxic growth. This knowledge will provide more 
understanding about C. glabrata physiology under hypoxic growth conditions which could 
ultimately be translated into the pathogenesis of this organism inside the host.

## Key facts

- **NIH application ID:** 10980498
- **Project number:** 5P20GM134973-05
- **Recipient organization:** UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
- **Principal Investigator:** Bao Gia Vu
- **Activity code:** P20 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $258,533
- **Award type:** 5
- **Project period:** 2020-03-01 → 2025-08-19

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10980498

## Citation

> US National Institutes of Health, RePORTER application 10980498, Transcriptomic and proteomic analyses of Upc2A under Candida glabrata hypoxic growth (5P20GM134973-05). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10980498. Licensed CC0.

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