# Regulation of membrane trafficking by Coronins

> **NIH NIH R01** · WASHINGTON UNIVERSITY · 2024 · $78,627

## Abstract

ABSTRACT
The long-term goal of the parent grant (1R01GM138448-01A1) is to get a better understanding
of how the extensive family of the Coronins transduces cellular signaling cues into actin network
remodeling to support cell migration, intracellular transport and cytokinesis. Coronins are bona
fide actin-binding proteins, however they do not directly alter actin filament dynamics. Instead,
they function as signaling scaffolds that recruit a host of other actin-remodeling and actin-
regulatory proteins that work in concert to alter actin network architecture and/or dynamics. Most
of our understanding of the Coronins stems from studies on the structurally and functionally
related short Coronins (Coro1-6), but next to nothing is known about the long or tandem Coronins
(Pod-1, Coro7). Leveraging the expertise of the lab in single molecule TIRF microscopy, we are
investigating how Coro7 regulates actin filament dynamics, directly as well as indirectly through
interaction with actin-regulatory proteins, including the other Coronins. Additionally, we have
performed an in-depth proteomic analysis to build the first Coro7 interactome and developed
several Coro7 CRISPR knockout cell lines. Combined with our insights gained from the
biophysical and biochemical characterization of Coro7, we will use these tools to determine how
Coro7 mechanistically regulates cell migration and intracellular trafficking, and by extension tumor
cell metastasis and invasion.

## Key facts

- **NIH application ID:** 10980713
- **Project number:** 3R01GM138448-04S1
- **Recipient organization:** WASHINGTON UNIVERSITY
- **Principal Investigator:** Silvia Jansen
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $78,627
- **Award type:** 3
- **Project period:** 2021-05-01 → 2025-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10980713

## Citation

> US National Institutes of Health, RePORTER application 10980713, Regulation of membrane trafficking by Coronins (3R01GM138448-04S1). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10980713. Licensed CC0.

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