# The role of stress physiology and reproductive hormones in the relationship between cannabis use and sleep in women of childbearing age

> **NIH NIH R01** · RUTGERS, THE STATE UNIV OF N.J. · 2024 · $443,263

## Abstract

ABSTRACT
This proposal aims to characterize the unique effect of recreational cannabis use on sleep in women. Sleep
was once believed to be a quiescent state, where self-reported measures might be sufficient; however,
biomedical technology advances now demonstrate that remarkable, essential biological processing occurs in
the brain and body throughout the night, including characteristic patterning of brain (EEG) and peripheral
(autonomic nervous system, HPA Axis) activity. Yet, the current void of scientific evidence on cannabis has led
to unsubstantiated marketing claims and public endorsement of cannabis as a sleep aid based nearly
exclusively on perceptions of improved sleep. Such claims carry specific health harms for women, whose
cannabis use may interact with a changing physiological context due to increases and decreases in
reproductive hormones. This application, submitted by an interdisciplinary sleep and addiction team, proposes
a 60-day prospective study with a within-subject design of 100 cannabis using, naturally cycling biological
females. It pairs sleep behavior with nocturnal physiology of brain and body systems to deconstruct the
proximal sleep effects of acute cannabis exposure at different phases of the menstrual cycle. Using multigroup
multilevel moderated mediation path analysis, stress-reactivity systems will be characterized as potential
mediators of the cannabis-sleep relationship. Framed through the lens of allostatic theory, this study views
repeated exposure to acute cannabis use episodes as stressors that can ‘pile up’ over time by increasing multi-
organ physiological load. Thus, apparent short-term improvements in sleep from cannabis use come at a cost
of wear and tear on multiple brain/body systems, potentially explaining the persistence of poor sleep outcomes
in individuals seeking treatment for or in recovery from addiction. The three study aims focus on comparing
sleep (self-report, actigraphy and EEG, Aim 1), HPA axis activity (cortisol, Aim 2) and autonomic nervous
system function (ECG, skin conductance, Aim 3) on nights following cannabis use to nights following no
cannabis use. To balance internal and external validity, recreational, non-daily cannabis users will self-
administer cannabis as usual with the exception that they self-purchase cannabis strains within a potency
range from legal state dispensaries and consume it with a cannabis flower vaporizer. Sleep and stress-
reactivity system data will be collected at home using FDA-approved wearable devices and saliva sampling.
Estradiol and progesterone will be assayed and used to distribute testing nights across the menstrual cycle;
menstrual phase will be tested as a moderator in Aims 1-3. This study addresses critical, and currently lacking,
information about women, a population that experiences more negative consequences from cannabis use and
a higher rate of sleep disturbances and disorders, yet has been largely overlooked in biological research due
to ...

## Key facts

- **NIH application ID:** 10985302
- **Project number:** 1R01DA061291-01
- **Recipient organization:** RUTGERS, THE STATE UNIV OF N.J.
- **Principal Investigator:** Jennifer F. Buckman
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $443,263
- **Award type:** 1
- **Project period:** 2024-09-01 → 2029-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10985302

## Citation

> US National Institutes of Health, RePORTER application 10985302, The role of stress physiology and reproductive hormones in the relationship between cannabis use and sleep in women of childbearing age (1R01DA061291-01). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10985302. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*