# Development of phosphorylation editing chimeras using kinase inhibitors

> **NIH NIH R21** · BROAD INSTITUTE, INC. · 2024 · $252,000

## Abstract

PROJECT SUMMARY
Protein post-translational modifications (PTMs) can activate, inhibit, or modify protein’s function. Small molecules
that inhibit enzymes that induce PTMs (e.g., kinases for phosphorylation) are available in abundance, but
methods to induce a given PTM of any protein on demand are challenging to develop. We propose to develop a
new class of small molecules which will induce protein phosphorylation by using a kinase inhibitor to recruit the
given kinase to phosphorylate a neo-substrate. These molecules will be formed by joining a small-molecule
kinase inhibitor with a small-molecule binder of the target protein so that an active kinase is brought into proximity
to the target protein to initiate phosphorylation.
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## Key facts

- **NIH application ID:** 10989139
- **Project number:** 1R21AI178690-01A1
- **Recipient organization:** BROAD INSTITUTE, INC.
- **Principal Investigator:** Amit Choudhary
- **Activity code:** R21 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $252,000
- **Award type:** 1
- **Project period:** 2024-06-17 → 2026-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10989139

## Citation

> US National Institutes of Health, RePORTER application 10989139, Development of phosphorylation editing chimeras using kinase inhibitors (1R21AI178690-01A1). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/10989139. Licensed CC0.

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