# Fast Multi-Functional 3D Imaging of Cellular Activities in Deep Tissue

> **NIH NIH R01** · UNIVERSITY OF ARIZONA · 2024 · $505,582

## Abstract

PROJECT SUMMARY
We propose to develop and disseminate an advanced 2p Bessel beam light sheet (2pBLS) microscopy
instrument for high-speed sub-micron-resolution volumetric imaging in deep tissue. The proposed instrument will
have a significantly higher volume imaging speed over a large field of view, maintain a sub-micron diffraction-
limited resolution in deep tissue despite the presence of tissue scatter and aberration, and perform simultaneous
lifetime imaging with FRET sensors and high-speed light sheet imaging of dynamics intensity-modulated sensors
for dual functional imaging in deep tissue. The system will be tested with two imaging studies in zebrafish and
mouse respectively. In zebrafish, we will demonstrate live FLIM imaging of genetically encoded FRET sensors
in zebrafish kidney and perform an imaging study of Ca2+ and cAMP activities in zebrafish renal cells. In mice,
we will demonstrate imaging of neural and glial activities genetically labeled with activity sensors (Ca2+ and GEVI),
FLIM-FRET sensors (PKA) and structural markers ex vivo and in vivo.

## Key facts

- **NIH application ID:** 10990024
- **Project number:** 1R01EB034921-01A1
- **Recipient organization:** UNIVERSITY OF ARIZONA
- **Principal Investigator:** Jun Ding
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $505,582
- **Award type:** 1
- **Project period:** 2024-09-01 → 2027-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10990024

## Citation

> US National Institutes of Health, RePORTER application 10990024, Fast Multi-Functional 3D Imaging of Cellular Activities in Deep Tissue (1R01EB034921-01A1). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10990024. Licensed CC0.

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