ABSTRACT: Education-Outreach Core The primary goals of the Education-Outreach Core are to promote a better understanding of issues concerning reproductive health and infertility in low income, underserved communities and by middle school, high school, and community college students. We propose to continue a two-pronged approach to education and outreach that we developed in the past grant period. First, we will continue and expand our collaboration with Urban Impact of Pittsburgh. A central part of Urban Impact’s mission is to provide youth with the education and skills to transition successfully from middle school to high school and into adult life. We will provide engaging half- day Saturday Academy style hands-on laboratory experiences to teach reproductive biology and genetics to high school students from Urban Impact (Aim 1a). We offered the “Sea Urchin Fertilization” lab and will continue to teach this lab moving forward. The students collected sperm and eggs from sea urchins, mixed them together in a petri dish, and used microscopes to visualize sperm swimming, sperm incorporation, sperm- egg fusion, pronuclear migration and the first cell divisions in the developing embryo. More recently, we offered a lab using worms (c. elegans) as a model to study genetics and meiosis. We have partially developed 3 new labs that will also be deployed for Aim 1a. As a result of participating in these highly successful lab experiences, youth at Urban Impact requested that our P50 program participate in their job training program (Jobs Inc.) so select students could gain further hands-on laboratory experiences while getting paid as part- time employees, which we propose in Aim 1b of this renewal. We will also make the labs we develop and refine available on a new website and at teacher workshops (Aim 1c). Second, we will continue and expand our collaboration with The DataJam, a nonprofit organization that runs a nationally available academic education program and competition to engage middle school, high school and community college students in learning data analytical skills and appreciating how valuable data and critical thinking skills are for virtually all career paths. DataJam teams formulate a research question, find publicly available data sets, analyze data and present their findings to a panel of judges. Over the past grant period P50 graduate students have developed DataSet Guides (that are freely available on the DataJam website) on “Male Reproduction & Infertility”, “Sexually Transmitted Diseases”, “Contraceptives”, “Gender Inclusive Health Care”, “Screening Newborns for Disease”. In this grant period we will develop new DataSet Guides on a variety of topics in reproductive biology and genetics (Aim 2a), train P50 students and fellows to become DataJam mentors (Aim 2b), and train teachers to guide projects in these areas (Aim 2c).