# Developing Novel Tools to Study Chemokine Receptor Ccr5 Expression and Function in Mice

> **NIH NIH R03** · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · 2024 · $84,500

## Abstract

PROJECT SUMMARY:
CCR5 is a chemokine receptor that holds significant importance in public health due to the following factors: 1)
CCR5 is the primary co-receptor for HIV infection; 2) approximately 1% of individuals with European ancestry
worldwide possess a natural deficiency in CCR5; 3) an FDA-approved CCR5 antagonist exists for HIV treatment
and is currently undergoing clinical trials for potential use in various chronic diseases. Our research has revealed
that the absence of CCR5 leads to heightened vulnerability to West Nile virus (WNV), a neurotropic flavivirus, in
both human subjects and mouse models. Additionally, we and other researchers have observed a similar
susceptibility to other neurotropic pathogens, such as Japanese encephalitis virus, tick-borne encephalitis,
cerebral malaria, and toxoplasma gondii. While these studies have been conducted using mice deficient in Ccr5,
further in-depth investigations have been limited by the lack of appropriate tools for mouse experimentation.
Through this proposal, our aim is to create a floxed Ccr5 reporter mouse and develop a collection of specific
antibodies targeting Ccr5. These resources will facilitate research on CCR5's function, expression patterns, and
its involvement in various inflammatory conditions and diseases.

## Key facts

- **NIH application ID:** 10993369
- **Project number:** 1R03AI182613-01A1
- **Recipient organization:** ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
- **Principal Investigator:** Jean Kyou Lim
- **Activity code:** R03 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $84,500
- **Award type:** 1
- **Project period:** 2024-06-01 → 2026-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10993369

## Citation

> US National Institutes of Health, RePORTER application 10993369, Developing Novel Tools to Study Chemokine Receptor Ccr5 Expression and Function in Mice (1R03AI182613-01A1). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10993369. Licensed CC0.

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