# MFB: Fluidics system for efficient site-specific labeling of RNAs

> **NIH NIH R01** · UNIVERSITY OF MASSACHUSETTS AMHERST · 2024 · $413,250

## Abstract

PPROJECT SUMMARY (See instructions):
mRNA in cells contains base modifications, strategically (and dynamically) placed at specific position in
the RNA. For fundamental research, RNA containing site-specific modifications can be generated by
chemical synthesis, but only at very short lengths. The proposed research aims to build on recent
advances in co-immobilized flow transcription to develop an enzymatic system to incorporate (multiple
and different) modified bases site-specifically. Such a system would enable more detailed studies of
translation and alternative splicing, for example, where base modifications are known to play key roles.
Synthesis of RNA with position-specific substitutions will also facilitate studies of RNA structure and
dynamics, where fluorescent probes for FRET/environment studies or stable isotopes for NMR
measurements are essential. A better understanding of the roles of specific base modifications will
ultimately inform future RNA therapeutics.

## Key facts

- **NIH application ID:** 10995397
- **Project number:** 1R01HG013861-01
- **Recipient organization:** UNIVERSITY OF MASSACHUSETTS AMHERST
- **Principal Investigator:** Craig T Martin
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $413,250
- **Award type:** 1
- **Project period:** 2024-07-01 → 2027-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10995397

## Citation

> US National Institutes of Health, RePORTER application 10995397, MFB: Fluidics system for efficient site-specific labeling of RNAs (1R01HG013861-01). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10995397. Licensed CC0.

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