Project Summary The 2024 FASEB NAD Metabolism and Signaling Meeting serves to unite researchers and clinicians who share the desire to elucidate NAD-dependent mechanisms and to evaluate the potential for NAD-based approaches for improving human health. This meeting overcomes barriers that inevitably form around sub- disciplines and promotes new collaborations. The meeting attracts attendees who work in different fields and retains a clear focus and interactive climate. This promotes genuine discourse, sincere collaboration, sharing of unpublished data, and exchange of burgeoning ideas. In turn, this meeting has accelerated the pace of discovery, expansion of NAD research into new areas, and resulted in forward progress of the field. In 2024 we seek to accommodate this growing and dynamic field, to push for technical breakthroughs, to obtain clarity in measurement standards and evaluation criteria for human trials, and to welcome investigators from new areas of research into our community. We are hosting new topic-specific sessions for cancer and neurobiology, and will pilot a pre-meeting event for trainees called Precursors. We are also hosting a dedicated speaker session for clinical trials, as well as a professionally moderated panel with the objective to develop discourse and consensus around how to approach studies of NAD in human health. The breadth of this meeting encompasses a vast diversity of biological topics and diseases, as well as includes the entire NAD metabolome such as NAD+, NADH, NADP+, NADPH, NaADP, ADP-Ribose, cyclic ADPR, Methylated-Nam, and pyridone nucleotides. The meeting further hosts researchers who use approaches that scale from atomic structural analyses to human physiology. Represented fields include chemistry, biochemistry, molecular and cell biology, preclinical organismal models, synthetic biology, engineering, and human trials. Thus, the major benefits of this meeting are the integration of ideas and collaborative outcomes across disciplines, as well as clarity and leadership for standards in NAD research.