# Monitoring of Oxygen Delivery and Tissue Oxygenation Deprivation in Critically Ill Neonates Using Resonance Raman Spectroscopy

> **NIH NIH R43** · PENDAR TECHNOLOGIES, LLC · 2024 · $318,724

## Abstract

Summary/Abstract
Shock physiology, from any cause, is characterized by an inability to deliver sufficient oxygen to the
tissues to meet cellular metabolic demands. Unfortunately, tools that assess the adequacy of oxygen
delivery in neonatal critical care patients are often invasive, imprecise, intermittent, and may be
misleading. For example, a patient with significant microvascular shunting may exhibit a falsely
reassuring arterial oxyhemoglobin saturation or normal lactic acid levels. Still, early identification of the
shock state is critical to the appropriate triage, resuscitation, and treatment of patients. Here, we propose
the advanced development and testing of an optical probe that monitors peripheral tissue oxygenation
as a marker of systemic hemodynamics with strong correlation to central venous oxygen saturation.
Local, capillary-ShbO2 (called StO2), primarily reflects the venous or post-O2 extraction compartment of
the vasculature and, thus, reflects the adequacy of oxygen delivery to tissues. Changes to systemic blood
supply, for example due to bleeding, infection, or low cardiac output may not change arterial oxygen
saturation, but are quickly reflected in peripheral StO2 as total oxygen delivery is reduced or peripheral
capillaries are shunted to maintain systemic perfusion.
We have developed a method for measuring StO2 based on resonance Raman Spectroscopy
(RRS). RRS takes advantage of resonant enhancement of the vibrational spectra from hemoglobin when
excited using a low power laser near a strong absorption peak (405nm). Thus, the StO2 measurement
can be taken against a tissue background and is insensitive to factors such as subcutaneous fat and skin
color. Using a prototype device in animal models, we have demonstrated the utility and feasibility of
measuring StO2 from the esophagus as a predictor of decompensation due to hemorrhage. In addition,
we confirmed the safety of long term use of the device. Here, we propose improvements to our sensor
which will enable measurements in a wider range of tissues and will prepare us to begin clinical trials in
a neonatal critical care patient population.

## Key facts

- **NIH application ID:** 11007510
- **Project number:** 1R43HD116647-01
- **Recipient organization:** PENDAR TECHNOLOGIES, LLC
- **Principal Investigator:** John Nagi Kheir
- **Activity code:** R43 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $318,724
- **Award type:** 1
- **Project period:** 2024-09-01 → 2025-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/11007510

## Citation

> US National Institutes of Health, RePORTER application 11007510, Monitoring of Oxygen Delivery and Tissue Oxygenation Deprivation in Critically Ill Neonates Using Resonance Raman Spectroscopy (1R43HD116647-01). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/11007510. Licensed CC0.

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