OVERALL PROJECT SUMMARY Alaska INBRE enhances capacity to conduct biomedical and behavioral health training and research within its network of the three universities of the University of Alaska system, the tribally-led Iḷisaġvik College, Southcentral Foundation, and Alaska Native Tribal Health Consortium. AK INBRE continues with its One Health research theme that emphasizes the importance of recognizing the interconnections among the health of people, communities, animals, and their shared environment in the rapidly changing state of Alaska. The AK INBRE Administrative Core coordinates and leads the network-wide annual research conference, proposal and career development workshops, and offers research administration advice to other NIH capacity building programs in Alaska. The Management Advisory Committee of network representatives contributes to AK INBRE management decisions for the network. The Steering Committee of senior leadership from the network institutions offers advice and counsel to the AK INBRE leadership on program foci, effectiveness, and impact including responses to program challenges. The Developmental Research Project Program awards for Pilot and Research Projects prioritize applications by investigators emphasizing One Health, as positively peer- reviewed by external experts and recommended by a national External Advisory Committee. The Research Experiences Core supports a student research program with research internships and career development opportunities for undergraduate and graduate students in addition to training opportunities in data science, analytic chemistry, genomics, biomarker development, and community based participatory research (CBPR), towards preparing a growing pipeline of students in Alaska entering into biomedical research careers. Research support in the Bioinformatics Core promotes data science, the research analytics for genomic, chemical, and stable isotope analytics, and the CBPR for community engaged research for investigators throughout the AK INBRE network in their development towards sustainable independent grant support as well as engaging students at their home institutions. The Sustaining Research Excellence Core is especially important for CBPR in the engagement of communities and local leadership together with investigators in the selection and approach of research questions and the dissemination of results. The INBRE program has been and will remain crucial to the success of students, faculty, and investigators in the ecosystem of biomedical and health research in Alaska.