Simeon Brown, Phase’s senior technician for the last two years, has been instrumental in our company’s technical success and will play an integral part not only in our Phase II grant but also in developing the company’s 3D printing commercialization capabilities beyond the timeline of this grant. Brown in his role plays a key part in developing the process and parameters of our 3D printing platform; performs experiments to understand the relationship between the materials and the structure in the 3D PDMS system; fine-tunes parameters that ensure dimensional accuracy of the microfluidic devices; and oversees all the mechanics in the design of Phase’s new 3D printing of microfluidic platforms. In the coming year, he will further refine his skills using robotics to develop full laboratory automation for device interface and creation using new hardware we will purchase. Brown is regularly developing novel approaches to develop the technology through his use of optical microscopes, plasma cleaners, and stereolithography 3D printers while honing his skills in software that include Autodesk Fusion and imaging processing software of Olympus microscopes. Brown will be instrumental in carrying out the remainder of the grant. Building upon our successful Phase I effort — during which we demonstrated the ability of our patent-pending 3D PDMS process to 3D print MF devices from conventional PDMS — this Phase II effort focuses on developing a pilot-scale commercial 3D PDMS system and using the 3D PDMS process to fabricate cutting edge in vitro blood-brain-barrier models for testing by our collaborators at Virginia Tech. They recently developed a MF BBB model containing a nanofiber basement membrane mimic which demonstrates a superior ability to recapitulate the in vivo BBB architecture. In Phase II, the team will optimize the architecture of the nanomembranes and then design and demonstrate a commercially producible 3D PDMS MF nanomembrane BBB model with integrated electrodes. We will also collaborate with the Nadkarni group at Harvard MGH to characterize the PDMS curing kinetics in 3D PDMS printing using laser speckle rheology. Aim 1: Operational Pilot-Scale 3D PDMS System. The objective of this aim is to design and a build pilot-scale 3D PDMS system. Milestone 1A: 3D PDMS Simulation & Model Accurately Predict Curing within +/-10%; Milestone 1A: 3D PDMS Simulation Model Accurately Predicts Curing within +/-10%; Milestone 1B: 3D PDMS unit achieves 200 mm3 /hr build rate for MF device. Aim 2: 3D Printed Nanofiber Blood-Brain-Barrier Model. The objective of this aim is to 3D print a highly reproducible BBB model which incorporates a nanofiber membrane and integrated TEER electrodes. Milestone 2A: Transport master curves for nanofiber membranes developed; Milestone 2B: Optimized nanofiber BBB model demonstrated by a 20% increase in TEER values for a coculture sample as compared to a monoculture sample.