# Mechanisms of cohesin regulation in vertebrates

> **NIH NIH R35** · OKLAHOMA MEDICAL RESEARCH FOUNDATION · 2024 · $15,949

## Abstract

Abstract
In vertebrate cells the cohesin protein complex plays critical roles in nuclear structure and
function. It tethers together the identical products of DNA replication, called sister chromatids,
until cell division and it also mediates intra-chromosomal bridging interactions, forming
chromosome loops and domains. While cohesion between sister chromatids is critical for
accurate chromosome segregation and certain kinds of DNA repair, the compaction of
chromosomes into loops and domains is essential for proper transcription and normal
development. How these different kinds of cohesion differ at the molecular level, the
mechanisms that ensure each outcome, and the amount of overlap between them are not well
understood. Using molecular genetic approaches, we are testing the impacts of specific
interactions, in multiple experimental systems, on cohesin regulation. In this application we are
requesting supplemental funds to purchase an ultra-low (-80) freezer in which to store strains,
cell lines, and proteins. Our current freezer has broken down and can no longer be repaired.
This freezer is essential for us to complete the projects described in the parent proposal.

## Key facts

- **NIH application ID:** 11022189
- **Project number:** 3R35GM149343-02S1
- **Recipient organization:** OKLAHOMA MEDICAL RESEARCH FOUNDATION
- **Principal Investigator:** Susannah Rankin
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $15,949
- **Award type:** 3
- **Project period:** 2023-04-03 → 2028-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/11022189

## Citation

> US National Institutes of Health, RePORTER application 11022189, Mechanisms of cohesin regulation in vertebrates (3R35GM149343-02S1). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/11022189. Licensed CC0.

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