Project Summary Under the current NIGMS R15 research award (1R15GM147857-01), this proposal requests supplemental equipment funds that will be used to replace an 11-year-old broken incubator for the cultivation of our genetic model system (C. elegans). The parent project focuses on the Proteolytic Regulation of Centrosome Proteins. The requested equipment is essential for carrying out the experimental plans for the genetic and molecular analyses using the in vivo model system. This administrative supplement fund will allow us to purchase a temperature- controlled incubator to precisely monitor the temperature-sensitive effects of mutant strains. This new imaging system will replace a broken incubator, which will serve a critical role in the success of the parent R15 project.