Administrative Supplement to Purchase Thermocycler Instruments ABSTRACT This supplement requests replacement of thermocycler equipment to support research funded through NIGMS grant R35 GM122532 (PI: Weeks). Our research program is focused on using genomics methods to understand the structure, function and ligandability of RNA secondary and tertiary structures. Methods and technologies invented in our laboratory are extensively adopted by research labs and biotechnology companies worldwide. RNA genomics methods ultimately all rely on massively parallel sequencing for their readout, and library preparation for sequencing requires multiple PCR or RT-PCR steps. Two of our thermocyclers have recently broken and are unrepairable, severely compromising our workflow. Procurement of these replacement instruments is essential to accomplish the core aims of the parent project, will accelerate biomedical research, and will enhance sustained biomedical impact by the Weeks laboratory.