Project Summary The primary goal of our funded research (R01GM141242) is to understand how the chromosome is organized and segregated during the cell cycle. We focus on the molecular mechanisms of SMC condensin complex, which is a key player for chromosome dynamics from bacteria to humans. Our projects combine genetic, molecular, cytological, and biochemical approaches. The Aim 2 of this grant is to understand the mechanism by which SMC is unloaded from the chromosome by its unloader, XerD. To address this question, we use purified proteins and investigate protein-DNA and protein-protein interactions. For our protein purification and protein interaction assays, a chromatography system is an essential piece of equipment. In the past, we had been using an AKTA chromatography system in our collaborator Dr Martha Oakley’s lab. However, Dr Oakley has closed her research lab and this equipment is no longer available to us. Since the biochemical assays are critical to the progress of my R01 grant, in this application for Administrative Supplements for Equipment, I am requesting funds to purchase an AKTA chromatography system (and necessary parts) for my own lab. Moreover, this proposed equipment has features that can expand our biochemical assays and allow us to adapt new methods to advance the parent grant.