PROJECT SUMMARY This Administrative Supplement is for the purchase of a CEM LibertyPrime2.0 microwave-assisted peptide synthesizer designed for Fmoc-based solid-phase peptide synthesis. The LibertyPrime2.0 uses a single mode self-tuned microwave reactor that drastically reduces amino acid coupling times while increasing coupling yields. A fiber optic temperature probe and reaction vessel camera within the sealed microwave cavity also ensure accurate temperature monitoring and complete waste removal during coupling and deprotection cycles. The LibertyPrime2.0 can efficiently synthesize long peptide fragments of up to 48 amino acids that are inaccessible by manual peptide synthesis. It is critical for our proposed studies to investigate the biophysical and biochemical effects of histone sumoylation and p53 methylation in human chromatin. This supplement seeks to replace an existing Liberty Blue peptide synthesizer that is no longer functional, which significantly limits our experimental capabilities. Use of the proposed instrumental setup will enable the semisynthesis of site-specifically modified histones and transcription factors, which will lead to the identification of novel pathways underlying neoplasia. This will reveal new therapeutic targets in a wide range of human cancers associated with the dysregulation of histone and p53 modifications.