# Preparative binary pump HPLC system with UV and refractive index detector capabilities

> **NIH NIH R15** · MICHIGAN TECHNOLOGICAL UNIVERSITY · 2024 · $77,283

## Abstract

Our NIH-funded project (1 R15GM152969-01) focuses on synthesizing and characterizing
fluorescent probes operating in the red to near-infrared region to measure pH levels within
organelles. The current synthesis involves multiple steps, with silica gel chromatography causing
delays and inefficiencies. The proposal suggests acquiring a preparative high-pressure liquid
chromatography (HPLC) system to improve purification at each step, addressing the limitations
of traditional silica gel column chromatography. The decision is driven by the complexity of the
probes and the need for enhanced resolution in separating closely related compounds. The shift
to preparative HPLC is expected to streamline the synthesis process and improve the overall
efficiency of the project.

## Key facts

- **NIH application ID:** 11034415
- **Project number:** 3R15GM152969-01S1
- **Recipient organization:** MICHIGAN TECHNOLOGICAL UNIVERSITY
- **Principal Investigator:** Rudy Lin Luck
- **Activity code:** R15 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $77,283
- **Award type:** 3
- **Project period:** 2023-09-18 → 2026-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/11034415

## Citation

> US National Institutes of Health, RePORTER application 11034415, Preparative binary pump HPLC system with UV and refractive index detector capabilities (3R15GM152969-01S1). Retrieved via AI Analytics 2026-05-27 from https://api.ai-analytics.org/grant/nih/11034415. Licensed CC0.

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