# Light scattering device for the development of protein clouds

> **NIH NIH R01** · UNIVERSITY OF OKLAHOMA · 2024 · $72,100

## Abstract

Project Summary
The supplement fund is requested under PA-20-272, NOT-OD-24-048, and NOT-GM-24-021 to purchase a
light scattering device, which will support NIGMS R01GM152704-01. The proposed research in R01GM152704
develops a new gene and drug delivery approach by activating biogenic nanoparticle complex, also known as
protein clouds, using ultrasound remotely to deliver Cas9-ribonucleoproteins safely and efficiently. After we
isolate and synthesize gas vesicles (GV) and protein clouds for delivery, we will measure hydrodynamic radius
and z-potential of protein clouds and individual GVs to confirm their size and tendency to aggregation. We
require reliable light scattering device, capable of determining the size of particles using dynamic light
scattering (DLS) and understanding sample stability using electrophoretic light scattering (ELS). The requested
state-of-the-art and single laboratory light scattering device will allow us to facilitate the successful
development of the proposed approach.

## Key facts

- **NIH application ID:** 11034458
- **Project number:** 3R01GM152704-01S1
- **Recipient organization:** UNIVERSITY OF OKLAHOMA
- **Principal Investigator:** Sangpil Yoon
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $72,100
- **Award type:** 3
- **Project period:** 2024-02-01 → 2029-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/11034458

## Citation

> US National Institutes of Health, RePORTER application 11034458, Light scattering device for the development of protein clouds (3R01GM152704-01S1). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/11034458. Licensed CC0.

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