# Resolving the intoxication mechanism of botulinum neurotoxins using single molecule structural biology

> **NIH NIH R01** · STATE UNIVERSITY NEW YORK STONY BROOK · 2024 · $107,410

## Abstract

Abstract
We are requesting an Administrative Supplement for Equipment Purchases to obtain an AKTA Pure 25M
Fast Protein Liquid Chromatography (FPLC) System to replace our failing AKTA Purifier FPLC system
installed in 2007. The requested instrument meets all of our specific needs and will maintain
compatibility with our existing chromatography media and methods. The AKTA Pure 25M is capable of
detecting multiple wavelengths, which is essential when working with the fluorescent dyes needed for
single molecule experiments. The proposed system is capable of the full range of chromatography
methods in particular the low pressure columns we use for small scale purifications. The ability to
generate recombinant protein samples of high purity and then separate them from fluorescent dyes
forms the basis of all experiments under the parent award. This award was funded for 4 years starting
09/20/2023.

## Key facts

- **NIH application ID:** 11034543
- **Project number:** 3R01GM151334-02S1
- **Recipient organization:** STATE UNIVERSITY NEW YORK STONY BROOK
- **Principal Investigator:** Mark E Bowen
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $107,410
- **Award type:** 3
- **Project period:** 2023-09-20 → 2027-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/11034543

## Citation

> US National Institutes of Health, RePORTER application 11034543, Resolving the intoxication mechanism of botulinum neurotoxins using single molecule structural biology (3R01GM151334-02S1). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/11034543. Licensed CC0.

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