# The interplay of TIMELESS and PARP1 in DNA replication fork stability

> **NIH NIH R01** · STATE UNIVERSITY NEW YORK STONY BROOK · 2024 · $170,657

## Abstract

ABSTRACT
We are requesting an instrument supplement to purchase an Incucyte S3 Live-Cell Analysis System from
Sartorius. Progress from the funded NIGMS R01 project for the past years has necessitated us implementing
the real-time and quantitative analysis of genome maintenance mechanisms in live cells. The Incucyte S3 allows
us to collect and analyze images of cell behaviors automatically and continuously in physiologically relevant
conditions. The specific applications relevant to the current project include, but are not limited to, real-time cell
cycle analysis, cellular proliferation, cell viability, and cell fate decision affected by the multiple mutants that we
have generated and under chemotherapy. The ability to monitor and image a variety of cellular processes
involved in DNA replication and stress responses under the real-time kinetics is crucial not only for the successful
completion of the parent R01 award but also for the new trajectories of research with a versatile and powerful
platform.

## Key facts

- **NIH application ID:** 11034675
- **Project number:** 3R01GM144399-03S1
- **Recipient organization:** STATE UNIVERSITY NEW YORK STONY BROOK
- **Principal Investigator:** Hyungjin Kim
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $170,657
- **Award type:** 3
- **Project period:** 2022-09-01 → 2026-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/11034675

## Citation

> US National Institutes of Health, RePORTER application 11034675, The interplay of TIMELESS and PARP1 in DNA replication fork stability (3R01GM144399-03S1). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/11034675. Licensed CC0.

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